Estimation of total cholesterol in serum by a micro method.

نویسندگان

  • K J CARPENTER
  • A GOTSIS
  • D M HEGSTED
چکیده

LEERS AND Lowa (1) HAVE PUBLISHED a method for the estimation of cholesterol on a micro scale in animal tissue by fluorometry involving the reaction of extracted cholesterol dissolved in trichloroethane with acetic anhydride and sulfuric acid. The present paper describes a simple and tested use of this reaction for the estimation of total cholesterol in 0.02 ml. of serum after extraction by a standard procedure (2) and without use of special apparatus.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

جایگزین درمانی هورمونی و تغییرات لیپوپروتئین ها در زنان یائسه

To evaluate the effectiveness of conjugated estrogen (Premarin) and progesterone in twenty-one postmenopausal women who had been menopause one year, we tested during a 6-month period the serum lipoprotein levels in subjects who offered by premarin in dosage of 0.625 milligram for days 1 to 25 and oral medroxy progestrone acetste for days 15 to 25 of a 30-day cycle. Twenty-one subjects completed...

متن کامل

The effect of wet cupping on serum lipid concentrations of clinically healthy young men: a randomized controlled trial

Objectives. Reduction in serum lipoproteins, especially LDL cholesterol, is a preventive approach against atherosclerosis. Bloodletting has been a recommended method to reduce serum lipoprotein concentrations. Present study was conducted to investigate the effects of wet cupping as a method of bloodletting on serum lipoproteins. In a randomized controlled trial, 47 men, 18-25 years old, without...

متن کامل

The effect of wet cupping on serum lipid concentrations of clinically healthy young men: a randomized controlled trial

Objectives. Reduction in serum lipoproteins, especially LDL cholesterol, is a preventive approach against atherosclerosis. Bloodletting has been a recommended method to reduce serum lipoprotein concentrations. Present study was conducted to investigate the effects of wet cupping as a method of bloodletting on serum lipoproteins. In a randomized controlled trial, 47 men, 18-25 years old, without...

متن کامل

Salvia officinalis (Sage) Leaf Extract as Add-on to Statin Therapy in Hypercholesterolemic Type 2 Diabetic Patients: a Randomized Clinical Trial

The efficacy and safety of Salvia officinalis combined with statin have not been evaluated in dyslipidemic diabetes mellitus type 2 (DDMT2) so far. The plant extract antioxidant activity was determined by the DPPH radical scavenging assay. The total flavonoid, total phenolic and quercetin contents of the capsules containing the plant extract were also measured. Moreover, the effects of 2-month ...

متن کامل

Serum Lipid Profile Alterations in Acute Leukemia Before and After Chemotherapy

Background: Serum lipids abnormalities have been observed in various forms of cancers including acute leukemia. Investigators report decreased total cholesterol and HDL, and elevated triglyceride in leukemic patients. Limited studies had been performed to discover the correlation between abnormal lipids profile and disease activity in leukemic patients in Iran. This study was done to evaluate t...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 3 4 Pt 1  شماره 

صفحات  -

تاریخ انتشار 1957